[Creative Biolabs] Protein Expression Kits

[Creative Biolabs] Products

[Creative Biolabs] Magic™ Cell-free Membrane Protein Expression Kits 제품 소개

Cell-free Membrane Protein expression은 대장균 용해물, 밀 배아 용해물, 식물 세포 용해물, 포유류 세포 용해물, 토끼 망상 적혈구 용해물, 곤충 세포 용해물과 같은 다양한 종의 세포에서 추출한 생체 분자 번역 기계를 사용하여 용액에서 재조합 단백질을 생산하는 것입니다. Creative Biolabs은 고품질의 대규모/중규모 막 단백질 발현을 위한 일련의 무세포 발현 시스템을 개발했습니다. Creative Biolabs는 글로벌 고객을 위해 최첨단 무세포막 단백질 발현 키트를 제공하고 있습니다.
 

• Facilitate the production of target proteins in high levels
• Simple protocol and easy setup with all necessary components
• The target proteins can be used in various downstream applications
• Protein of interest can be synthesized and visualized in a few hours
• Low cost

• Synthesized in as little as 1 hour and easy setup with all necessary components in a single-tube reaction
• Provides high yield and functional protein
• Enables the synthesis of large proteins (such as over 150 kDa)
• Modifications such as glycosylation and phosphorylation could be easily obtained
• Translation enhancement factor included in the Accessory Proteins ensures the high translation level
• Various applications: high throughput screening, synthetic biology, toxic or difficult to express protein synthesis, studies on protein folding, activity, and protein-protein interactions

Wheat Germ Lysate
 

• Membrane protein has been synthesized using proteoliposome
• No need to prepare reagents
• Reagents necessary for each reaction are already pre-packaged in tubes
• Synthesize proteins with just a few pipetting steps
• Target proteins can be confirmed through SDS-PAGE/CBB staining

Insect Cell Lysate
 

• Convenient, quick, single-tube, coupled transcription/translation system
• Allows both transcription and translation to occur in a single reaction
• Can be scaled by changing the component volumes proportionally
• T7 RNA polymerase promoter
• Addition of labels to the reaction

Rabbit Reticulocyte Lysate
 

• Compared with conventional cell-based/Cell-free Membrane Protein synthesis platforms, RRL system exerts multiple superior features
• Easily prepared
• Cap-independent translation
• Compatible with mammalian post-translational protein modification system
• Large proteins greater than 30 kDa can be expressed efficiently
• Compatible with high-throughput format for protein microarrays and other screening procedures

Plant Cell Lysate
 

• High-yield: Up to 3 mg/mL expressed protein
• Easy to use: One-step reaction protocol
• Fast: High yield in just 48 hours
• Flexible: Express "difficult to produce" proteins, cytotoxic proteins, etc.
• Convenience: suitable for various membrane protein expressions

 

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